![]() Have you found any related forum topics? If so, cross-link them. A Defined Methodology for Reliable Quantification of Western Blot Data.But since it is difficult to place the line on the bell-shaped curve exactly at the same spot it’s hard to tell if it overlapping boxes affect the quantified band intensities. Since then, it has been mentioned in the titles, abstracts, and keywords of more than 400,000 PubMed-listed publications. I quantified the bands separately and with overlapping boxes. The Western blot technique was first published 40 years ago. I don’t know if the overlapping of boxes of two bands affects the quantified values of each band ![]() Would this affect quantification considering that the white space around the two bands is overlapping but the overlapping boxes don’t reach over the actual band? To quantify my western blot bands: I need to place a box around each band Then go to Anayze>Gels>Plot lanes From the generated plots I mark the bell shaped curve with the line tool Use the wand and get a quantification value from the intensity of my band. Plots of CHEMI_10242021_154542_fis_br_se_cropped_FIS_marked_bell curves.tif (523.5 KB)Ĭan the boxes from different bands overlap? I need a certain box size to fit the largest band, but this leads to the box around one band overlapping in the white space of the box of the next band (not over the next band, only the white space). Hello, I have a question regarding western blot analysis using imageJ. *Use the wand and get a quantification value from the intensity of my band.ĬHEMI_10242021_154542_fis_br_se_cropped_FIS_marked.tif (65.6 KB) From the generated plots I mark the bell shaped curve with the line tool.Search 216,022,918 papers from all fields of science. Skip to search form Skip to main content Skip to account menu. I have a question regarding western blot analysis using imageJ. Semantic Scholar extracted view of 'A new background subtraction method for Western blot densitometry band quantification through image analysis software.' by G.
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